Heavy rain had left many inside Hampden Park soaked, but the inclement weather failed to dampen Bolt's spirits.
The sprint sensation was caught in controversy earlier this week for alleged derogatory remarks made about the Games being held in Glasgow.
But on the penultimate day on Saturday, he made the headlines for the right reasons when he dashed past England's Danny Talbot on the final stretch as Jamaica clocked a winning time of 37.58 seconds.
England had to settle for silver, while Trinidad and Tobago finished in the bronze-medal position.
Jamaica capped a superb evening of relay action by also winning the women's 4x100m and 4x400m races.
However, there was no clean sweep in the relays as England triumphed in the 4x400 men's race with a time of three mins, 46 secs.
Kenya had plenty of reason to celebrate at Hampden Park.
James Magut - a silver medalist in Delhi four years ago - triumphed in the 1500m ahead of compatriot Ronald Kwemoi, while Kenya also had a one-two in the women's 5000m - Mercy Cherono leading home Janet Kisa.
Julius Yego completed a fine evening for Kenya in the track and field as he threw 83.87m to win the men's javelin, while in men's triple jump Khotso Mokoena posted a jump of 17.20m to win gold ahead of Nigeria's Tosin Oke and India's Arpinder Singh.
The poor weather particularly affected the women's pole vault, but Australia's Alana Boyd overcame the difficult conditions to claim the gold medal.
England's Tom Daley was the clear victor in the men's 10m platform in diving.
The 20-year-old, who won two golds in Delhi in 2010, scored 516.55 points.
Esther Qin took first place for Australia in the women's 3m springboard diving final.
England's Nicola Adams added Commonwealth boxing gold to the Olympic title she won in London two years ago, defeating Northern Ireland's Michaela Walsh in the women's flyweight division.
Walsh's compatriot Paddy Barnes fared better by winning the men's flyweight, while countryman Michael Conlan won the bantamweight gold.
Scotland's Charlie Flynn and Josh Taylor brought joy for the home fans in the men's lightweight and light welterweight divisions respectively.
England took the lead in the women's hockey final, but Australia rebounded to level 1-1 before triumphing 3-1 in a shoot-out victory.
Nigeria took a clean-sweep of powerlifting golds, Esther Oyema and Loveline Obiji sealing success in the women's lightweight and the heavyweight events respectively, as Paul Kehinde and Abdulazeez Ibrahim won the men's lightweight and heavyweight medals.
India's Dipika Pallikal and Joshana Chinapp celebrated victory in the women's squash doubles, while at the Scotstoun Table Tennis arena Singapore celebrated gold in the men's singles courtesy of Zhan Jian and the nation also won the women's doubles.
In the mixed doubles Joanna and Paul Drinkhall triumphed in the all-England clash against Tin-Tin Ho and Liam Pitchford.