The new rules were confirmed in an AOC statement on Tuesday and also apply to next year's Winter Olympics in Sochi as well as Youth Games.
Under the new regime flagged last month by Rio de Janeiro Chef de Mission Kitty Chiller, Olympic team members are not allowed in the Olympic Village or other team locations if they are drunk and behaving in inappropriate ways.
The new rules follow the release of the Bluestone Review earlier this year, which found a 'toxic culture' in Australia's swim team at the London Games, which saw athletes abuse prescription drugs, alcohol and curfews.
Members of the men's 4x100m freestyle relay team including veteran Eamon Sullivan and hot shot James Magnussen faced a Swimming Australia integrity panel inquiry in February where they admitted to unauthorised alcohol and drug use in Manchester before the London Olympics.
They defended by their misbehaviour at the inquiry by saying their late-night parties were necessary for team bonding.
Boozing has also been banned on the team charter flight returning to Australia after the Games.
"These restrictions have been implemented to ensure that Australia's Olympic athletes are given the opportunity to compete to the best of their ability and with distinction," AOC president John Coates said.