First Seal was due to contest the Group II Tramway Stakes (1400m) at Randwick on Saturday but a recurring foot problem necessitated her withdrawal from the event at 7:08am.
An abscess in the same foot ruled her out of her initial first-up run in the Group II Warwick Stakes (1400m) at Randwick a fortnight ago.
The daughter of Fastnet Rock will undergo an MRI scan on Monday to determine the extent of the problem.