The Mozambique-born striker scored 733 goals in 745 professional matches and is considered as one of the sport's all-time greats.
Here we look at the reaction of the football world to the death of a man whose many achievements include finishing top scorer at the 1966 FIFA World Cup:
"Always eternal Eusebio, rest in peace" - Real Madrid and Portugal star Cristiano Ronaldo offers his tribute on Twitter.
"The king! Great loss for all of us! The greatest!" - Luis Figo, Portugal's most capped player, tweets his sadness at the news.
"Mourning the death of Eusebio, a brother to me. We became friends in England in the 1966 World Cup. I last saw him at the recent Brazil-Portugal match in Boston. My condolences to his loved ones. May God receive him with open arms" - Brazilian legend Pele offers his thoughts on Twitter.
"One of the greatest football players ever has passed away. My friend Eusebio died last night. My thoughts are with his family" - German football great Franz Beckenbauer shares his sorrow on Twitter.
"He was not only a great inspiration but also an important figure in upholding the values, principles and feelings of football, even after finishing his career. I think guys like him never die. History is there and history doesn't let them die. I knew him well. He enjoyed life. He leaves a great legacy, so rest in peace" - Portuguese coach Jose Mourinho salutes Eusebio.
"Eusebio was a football & FIFA ambassador. He'll be sorely missed. Rest in peace Black Panther" - FIFA president Sepp Blatter offers his condolences on Twitter.
"Sad news that Eusebio has died. One of the greats of his generation. Could play and strike a ball like very few others. #RIP" - England legend Gary Lineker takes to Twitter to hail Eusebio.
"Respect to a man who meant so much to the game I cherish. Eusebio, one of the all time greatest, may he Rest In Peace" - Manchester City and Belgium midfielder shows his respect on Twitter.
"Sad news that Eusebio has passed away, he was a true legend, a fantastic ambassador to football, a gentleman to the core. R.I.P. Sir" - Peter Schmeichel, who won 129 caps for Denmark, on Twitter.
"Sad to hear that Eusebio has passed away. 733 goals in 745 appearances will always mark him down as a true legend of the game. R.I.P" - Former England striker Michael Owen on Twitter.