The British pair saw off challenges from the United States and New Zealand to set a world's best time of six minutes, 50.61 seconds and defend their nation's title.
Glover won gold alongside alongside Polly Swann in Chungju last year and repeated the feat with Stanning this time after a race in which the Olympic gold medalists led from start to finish.
There was further British joy in the Men's Four as Pete Reed, Tom James, Alex Gregory and Andrew Triggs Hodge saw off the USA and Australia into second and third respectively.
New Zealand's Eric Murray and Hamish Bond also defended their Men's Pair gold from last year.
Murray and Bond finished in a time of six mins, 09.340secs but were made to work hard by the remainder of the field - only taking the lead after 1,000 metres.
However, from there, the Kiwis never looked like relenting as they surged to the finish line ahead of Great Britain in second and South Africa a place further back.
There was better news for the South African contingent in the Lightweight Men's Double Sculls with a world's best time of six mins, 05.360secs earning them gold.
James Thompson and John Smith broke new ground in a race that saw all boats within half a length of one another going into the final 500 metres.
Thompson and Smith were required to hold off late French pressure as Stany Delayre and Jeremie Azou were beaten into second - Norway's Kristoffer Brun and Are Strandli taking bronze.
Julia Edward and Sophie MacKenzie resisted the threat from Canadian pair Patricia Obee and Jennerich Lindsay on their way to gold in the Lightweight Women's Double Sculls.
China were a place further back before Germany took gold in the penultimate event of the day - the Women's Quadruple Sculls.
Following a positive start, Carina Baer, Tina Manker, Julia Richter and Stephanie Schiller never looked back as the Germans retained their gold from a year ago.
The Men's Quadruple Sculls wrapped up the day's racing in fine fashion as Ukraine edged out Great Britain to take first place.